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This Concept Map, created with IHMC CmapTools, has information related to: LAB 15 - ISOLATION AND IDENTIFICATION OF STAPHYLOCOCCI - IDENTIFICATION, Staphylococcus epidermidis mannitol salt agar Does not ferments mannitol: no acid produced, the phenol red pH indicator remains red (alkaline)., Staphylococcus aureus coagulase test Coagulase positive: the coagulase causes the inoculated citrated rabbit plasma to gel or coagulate., Staphylococcus epidermidis Staphyloslide® Latex Test Does not produce coagulase or Protein A: the latex particles coated with fibrinogen and IgG do not clump., Staphylococcus aureus mannitol salt agar Ferments mannitol: acid produced turns the phenol red pH indicator from red (alkaline) to yellow (acid)., Staphylococcus saprophyticus mannitol salt agar Usually ferments mannitol: acid produced turns the phenol red pH indicator from red (alkaline) to yellow (acid)., Staphylococcus saprophyticus blood agar with a Taxo NB® disc Gamma reaction (no lysis of the red blood cells around the colonies), resistant to novobiocin (Taxo NB® disc), usually no pigment., Staphylococci identification Staphylococcus saprophyticus, Staphylococci identification Staphylococcus aureus, Staphylococcus aureus blood agar with a Taxo NB® disc Beta hemolysis (complete lysis of the red blood cells around the colonies), sensitive to novobiocin (Taxo NB® disc), often a gold, water-insoluble pigment., Staphylococci microscopic appearance Gram-positive cocci most commonly in irregular grape-like clusters, Staphylococcus saprophyticus coagulase test Coagulase negative: the citrated rabbit plasma does not gel or coagulate., Staphylococcus epidermidis coagulase test Coagulase negative: the citrated rabbit plasma does not gel or coagulate., Staphylococci identification Staphylococcus epidermidis, Staphylococcus saprophyticus Staphyloslide® Latex Test Does not produce coagulase or Protein A: the latex particles coated with fibrinogen and IgG do not clump., Staphylococcus aureus Staphyloslide® Latex Test Produces coagulase and/or Protein A: the latex particles coated with fibrinogen and IgG clump., Staphylococcus epidermidis blood agar with a Taxo NB® disc Gamma reaction (no lysis of the red blood cells around the colonies), sensitive to novobiocin (Taxo NB® disc), usually no pigment.
